United Kingdom (UK)
Recombinant monoclonal antibody to vWF. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma F8/86.
UniProt Accession Number of Target Protein: P04275
Alternative Name(s) of Target: von Willebrand factor; von Willebrand antigen 2; von Willebrand antigen II; coagulation factor VIII VWF; factor VIII-related antigen; F8; F8VWF; VWD
Immunogen: This antibody was raised against von Willebrand factor isolated from human plasma.
Specificity: This antibody recognises human von Willebrand factor (also know as Factor VIII related antigen). It is produced by endothelial cells and megakaryocytes and is involved in the process of hemostasis by engaging in adhesion of platelets to the sites of vascular injury. Mutations in this gene lead to Von Willebrand's disease which causes frequent bleeding.
Application Notes: IHC: F8/86 antibody was used in immunohistochemistry expermients to stain against vWF in samples of murine stomach tissue in a study exploring the potential use of mesenchymal cells in myocardium regeneration (Freemen et al., 2015). ICC: This antibody was also used in an attempt to characterise human gingival microvascular endothelial cells where it identified vWF via immunocytochemical staining (DeCarlo et al., 2008). FACS: Another group has successfully used F8/86 antibody in a cell sorting procedure in a study which analysed the influence of physical environment on the rat mesenchymal stem cells (Xue et al., 2017).
Antibody first published in:
Naiem et al. The value of immunohistological screening in the production of monoclonal antibodies. J Immunol Methods. 1982;50(2):145-60. PMID:6806388
Note on publication:
This article describes generation of the anti-vWF antibody and its characterisation by immunohistological labelling.
Immunofluorescence staining of HepG2 cells using anti-vWF antibody (Ab01314) F8/86. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton and stained with the chimeric mouse IgG version of F8/86 (Ab01314-1.1) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01314-1.1, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody (Ab00178-1.1) followed by Alexa Fluor® 488
Flow cytometry using the Anti-vWF antibody F8/86 (Ab01314). Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (Ab00178-1.1; isotype control, black line) or the mouse IgG1 version of F8/86 (Ab01314-1.1, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.