United Kingdom (UK)
Recombinant monoclonal antibody to Yellow Fever Virus. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2D12.
UniProt Accession Number of Target Protein: P03314
Alternative Name(s) of Target: YFV; protein E; 17D; 17D YFV; Envelope protein E
Immunogen: The original mouse version of this antibody was raised by immunizing female Blab/c mice with the 17D YF vaccine.
Specificity: The antibody is specific for protein E of the 17D yellow fever virus. Protein E binds to host cell surface receptor and mediates fusion between viral and cellular membranes.
Application Notes: To characterize the mouse version of this antibody a radioimmunoassay with this antibody was performed on Vero cells infected with 17D YF. Further the presence of this antibody was confirmed by a 90% plaque reduction neutralization test in Vero cells (Schlesinger et al, 1983; pmid:6187129). To develop a diagnostic test to determine the presence of yellow fever virus in the blood of a patient. An ELISA was performed on the 17D YFV antigen using the mouse version of this antibody (Monath and Nystrom, 1984; pmid:6364854). To define the process of YFV entry into target cell. Flow cytometry was preformed using the mouse version of this antibody on cells infected with YFV. (Fernandez-Garcia et al, 2016; pmid:26861019). Type specific and cross reactive determinants were identified using immunofluorescence on yellow fever viruses using the mouse version of this antibody (Monath et al, 1984; pmid:6206738).
Antibody first published in:
Schlesinger et al. Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition, and immune precipitation of the virus envelope protein. Virology. 1983 Feb;125(1):8-17. PMID:6187129
Note on publication:
Nineteen monoclonal antibodies were produced to the 17D strain of yellow fever virus (17D YF).