United Kingdom (UK)
Recombinant monoclonal antibody to Zearalenone. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody QY1.5.
Alternative Name(s) of Target: RAL; F-2 toxin; F-2 mycotoxin; Mycotoxin F2; [6-(10-hydroxy-6-oxo-trans-1-undecenyl)-bresorcylic acid lactone]; CAS NO: 17924-92-4; ChEBI:10106
Immunogen: The original antibody was generated using phage display technology by panning an scFv library against zearalenone-ovalbumin conjugate. The scFv library was created by linking the heavy and light chain genes of antizearalenone hybridoma cell line (2G3-6E3-2E2) using DNA linker encoding peptide (Gly4Ser)3 and cloning into a phagemid (pCANTAB5E) to be expressed as a fusion protein on the surfaces of recombinant phages. The original hybridoma (2G3-6E3-2E2) was generated using zearalenone-BSA conjugate as an immunogen.
Specificity: This antibody is specific for zearalenone and show some cross reactivity with hydroxylated zearalenone analogs. It is a mycotoxin produced mainly by fungi belonging to the genus Fusarium in foods and feeds. Possess estrogenic activity in pigs, cattle and sheep, with low acute toxicity. When fed to animals, the compound causes hypoestrogenism with symptoms such as enlargement of the uterus and nipples, vulvar swelling, vaginal prolapse, and infertility
Application Notes: The binding specificity of this scFv antibody towards mycotoxin zearalenone was determined using ELISA. The scFv version of this antibody was also able to detect zearalenone in spiked corn extract in a competitive-indirect ELISA (PMID: 8979354).
Antibody first published in:
Yuan et al. Molecular cloning, expression, and characterization of a functional single-chain Fv antibody to the mycotoxin zearalenone. Appl Environ Microbiol. 1997 Jan;63(1):263-9. PMID:8979354
Note on publication:
Describes the generation of a functional scFv antibody having specificity for mycotoxin zearalenone using phage display technology.