New SARS-CoV-2 Neutralizing Antibodies Derived from COVID-19 Patients

Our catalog now includes SARS-CoV-2 neutralizing antibodies derived from individuals infected with COVID-19. The antibodies, originally generated by Fred Hutchinson Cancer Research Center, have been engineered into recombinant formats useful for COVID-19 research and diagnostic development.

The new antibodies were generated from the blood cells of an infected COVID-19 patient and have shown in a recent article to display neutralizing activity against SARS-CoV-2, the coronavirus that causes COVID-19. Two antibody clones (CV1 and CV30) each bound the SARS-CoV-2 spike glycoprotein, with CV30 also shown to bind the receptor binding domain (RBD) and inhibit the interaction with the host cell receptor ACE2. Both antibodies are now available in a variety of species, isotypes and subtypes designed to extend their usefulness in COVID-19 applications.

Binding curve of anti-spike protein (RBD) antibody CV30 (Ab02019-1.1) to SARS-CoV-2 spike protein RBD-Fc fusion protein. ELISA plate was coated with SARS-CoV-2 spike protein RBD-Fc rabbit fusion protein (Pr00444; Absolute Antibody) at a concentration of 2.5 µg/ml. A 3-fold serial dilution from 1,000 ng/ml was performed using a mouse IgG1 antibody Ab02019-1.1-HRP.

Available formats include:

• Human formats such as IgG1, IgG3, IgM and IgA, for use in neutralization assays and as serological controls in COVID-19 diagnostic tests
• Species such as mouse and rabbit for detection applications and co-labeling studies
• Fragment formats for better tissue penetration and in vivo imaging
• Engineered Fc Silent™ domains to facilitate research into antibody-dependent enhancement

As with all Absolute Antibody reagents, the new antibodies are recombinantly produced for ensured batch-to-batch reproducibility, high purity and low endotoxin levels. Learn more about each clone by following the links below.

The new neutralizing antibodies join our full collection of engineered coronavirus reagents, including the spike glycoprotein antibody clone CR3022, nucleoprotein antibodies, ACE2 Fc fusion proteins, and anti-human immunoglobulin antibodies for use in diagnostic tests. Contact us with any questions or to request a quote.